A procedure for the estimation over time of metabolic fluxes in scenarios where measurements are uncertain and/or insufficient

Table 1 Comparison between MFA and FSA

	Traditional Metabolic Flux Analysis (MFA)		Flux Spectrum Approach (FSA)
	Flux estimation	Consistency	Flux estimation	Consistency
Determined	Yes.	Consistency check χ².	Yes.	Consistency check χ².
Redundant		Flux adjustment.	Considers uncertainty.	Flux adjustment or use of a band of uncertainty.
			Detects sensitivity problems.
Determined	Yes.	No.	Yes.	Detect some inconsistencies.
Not Redundant		No.	Considers uncertainty.
			Detects sensitivity problems.
Underdetermined	No.	Consistency check χ².	Yes (not guaranteed).	Consistency check χ².
Redundant		Flux adjustment.	Considers uncertainty.	Flux adjustment or use of a band of uncertainty.
			Detects sensitivity problems.
Underdetermined	No.	No.	Yes (not guaranteed).	Detect some inconsistencies.
Not Redundant		No.	Considers uncertainty.
			Detects sensitivity problems.

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