Figure 2
Top: Blast alignments of contigs resulting from minimus assembly of Zebrafish shotgun reads to the human GPC3 protein. The top four matches correspond to contigs that do not have any significant similarity to each other at the nucleotide level, indicating the presence of at least 4 homologues to the GPC3 gene in the Zebrafish genome. Bottom: Alignment of the Zebrafish GPC3 protein to the human GPC3 protein highlighting that the minimus assembly covers the majority of this gene.