Figure 1
From: Model based analysis of real-time PCR data from DNA binding dye protocols
Models for PCR amplification efficiency. The effective amplification efficiency for each PCR cycle was calculated as Tn+1/T n – 1, where T n and Tn+1were the PCR product yield at cycles n and n+ 1 respectively. Data points are the effective amplification efficiency vs. PCR product yield from a representative PCR reaction performed in triplicate. Lines are the fit of models 1 (A), 2 (B) and 3 (C) to the experimental data. Inserts are the residuals for each fit. The determination coefficient (R2), corrected Akike's Information Criterion (AIC) and the best fit value for Ei ± asymptotic standard error are shown in the graphs. Ei for model 3 was calculated from Eq. (3). Model 1 was fitted by linear regression, while models 2 and 3 were fitted by non-linear regression.