Model based analysis of real-time PCR data from DNA binding dye protocols

BMC Bioinformatics

Table 1 Estimation of model parameters.

Estimation of T₀, b and Tm by fitting Eq. (1) and (2) to experimental data (A), and estimation of T₀ by fitting Eq. (1) and (2) to experimental data using b and Tm values previously obtained from the fit of Eq. (2) to experimental amplification efficiencies (B). Shown is the dilution of mouse midbrain cDNA used for each real-time PCR run, the best fit values for T₀, the asymptotic estimation of the standard error (SE), and the T₀ mean value ± standard error of the mean (SEM). For (A), the correlation between T₀ and the other parameters estimated by non-linear regression is also shown.

ISSN: 1471-2105