Figure 4

(a) Collection of toggle signals from the captured pseudo-aptamer. The molecule appears as the one in Fig. 3(a), unattached to the 5 nucleotide ssDNA. (b) Collection of toggle signals from the pseudo-aptamer + 5 nucleotide ssDNA. The bound complex appears as in Fig. 3(a), with the 5 nucleotide sequence joined to the overhang section. One distinctive change only observed in the blockade signals after binding agent introduced, aside from the level dwell-time changes, are the much higher frequency upward "spike" transitions, from the lower level to the upper level.