Figure 2

Linked Antibody Experiment [10]. Approximately shown to scale on the left is the DNA-antibody complex and its intended captured orientation in the alpha-hemolysin channel. An internal amino thymine modification or phosphate backbone modification with primary amine on a six carbon spacer arm is crosslinked using 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) to the peptide carboxyl terminus of the antibody heavy chain. This crosslinkage results in a covalent bond between the primary amine and the carboxyl terminal.