Spike-in expression ratio estimates. Reconstructed expression ratio estimates of spikes 7 (+ markers) and 8 (x markers) are plotted for lmbr/lmbr_dye/limmaQual (solid line), anovaFix (dotted line), and anovaMix (dashed line). Concentrations (cpc; copies per cell) of 104 cpc (panel A), 10 cpc (panel B), and 10-1 cpc (panel C) were used as reference points. Estimated ratios were sorted from low to high concentrations. The solid grey line (o-markers) corresponds to the expected ratios for the known concentrations.