Figure 7
From: ComPath: comparative enzyme analysis and annotation in pathway/subsystem contexts
Case II: isozymes. EC 2.7.1.11 and six gamma-proteobacteria species were selected: Escherichia coli K-12 MG1655 (eco), Escherichia coli O157 EDL933 (ece), Salmonella typhi CT18 (sty), Salmonella enterica serovar typhi Ty2 (stt), Shigella flexneri 301 (sfl), Shigella flexneri 8401 (sfv). Initial pathway reconstruction detected no phophofructokinase gene in Shigella flexneri 8401 (sfv). BAG clustering clearly divides class I and class II isozymes from five species. To detect two isozymes classes in Shigella flexneri 8401, two sequential CSR-HMM searches using two set of protein sequences as queries detect SFV_3578 (class I) and SFV_1498 (class II).