Figure 6
From: An efficient visualization tool for the analysis of protein mutation matrices
Mutation patterns in CDRs and FRs of high-affinity antibody sequences. The matrices shown were generated from the analysis of affinity-matured anti-thyroid peroxidase antibodies (anti-TPO, KD = 10-9) derived from six different germlines. No distinction between mutations in light and heavy chains were made. The colored spots indicate the positions of artificially-introduced mutations in engineered antibodies that were associated with decreased affinity (Table 4); each mutation is indicated in two different matrices, since the exposure patterns of these residues were not indicated in the original references. These mutations were never observed for high-affinity antibodies. Unscaled, grayscale matrices were used to improve contrast.