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Table 2 t statistic values obtained for all variable combinations.

From: A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition

 

Amplification mix percentage

Log 10 input DNA

100%

90%

80%

70%

60%

7.5

0.28348

1.15431

2.9303*

5.43493**

4.26067**

6.5

-3.0233*

-0.5329

7.8552**

8.68609**

7.28178**

5.5

-2.2195*

2.70419*

4.7185**

8.61406**

4.60465**

4.5

0.97856

1.32162

2.34*

16.5192**

17.5903**

3.5

1.00647

-1.038

2.3307*

13.2572**

4.65683**

2.5

-1.731

-0.5995

5.8385**

6.90378**

6.13465**

1.5

0.14417

1.25452

-0.898

1.87978

3.69668**

  1. When t < 0 the Richards coefficient is lower than 1, while for t > 0 the Richards coefficient is higher than 1. Significance levels: * 0.05 <p < 0.01; ** p < 0.01