Figure 2

An illustration of unmethylated and methylated model construction in MLDA in A2780 cell line. a: Three patterns can be observed on the scatter plot of log-transformed Cy3 (undigested) against log-transformed Cy5 (digested) intensities. b: The unmethylated model constructed using 94 mitochondrial sequences as a unmethylation reference. c: The intermediate model constructed through the 97.5 quantile residual. The point X is the 97.5 quantile residual. The microarray probes colored in blue (standardised residual to the intermediate model is less than 2) are selected to construct the methylated model. d: Methylated (in blue) and unmethylated (in red) models in A2780 cell line.