Skip to main content

Table 2 Results of experimental validation of predicted long and short, polymorphic and monomorphic EST-SSRs of potato

From: Large-scale identification of polymorphic microsatellites using an in silico approach

Number of SSRs

long poly2a

short poly2b

long mono3a

short mono3b

total

for which primers were designed

25

25

15

15

80

with no or not clear products

2

4

6

1

13

with products more than 500 bp

2

2

1

2

7

that produced scorable markers1

21 (84%)

19 (76%)

8 (53%)

12 (80%)

60 (75%)

N of polymorphic SSRs

21 (100%)

18 (95%)

7 (88%)

10 (83%)

56 (93%)

  1. 1Marker are considered scorable when they produced amplicons of less than 500 base pairs
  2. 2 EST-SSRs classified as polymorphic by PolySSR; 3 EST-SSRs classified as monomorphic by PolySSR; along SSRs: at least 10 repeats in repeat motif for dinucleotide SSRs, 6 for tri-SSRs, 5 for tetra-, penta- and hexa-SSRs; bshort SSRs: at most 5 repeats for dinucleotide SSRs, 4 for tri-, tetra-, penta- and hexa-SSRs (see Materials and Methods).
Back to article page

BMC Bioinformatics

ISSN: 1471-2105

Contact us