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Table 2 Results of experimental validation of predicted long and short, polymorphic and monomorphic EST-SSRs of potato

From: Large-scale identification of polymorphic microsatellites using an in silico approach

Number of SSRs long poly2a short poly2b long mono3a short mono3b total
for which primers were designed 25 25 15 15 80
with no or not clear products 2 4 6 1 13
with products more than 500 bp 2 2 1 2 7
that produced scorable markers1 21 (84%) 19 (76%) 8 (53%) 12 (80%) 60 (75%)
N of polymorphic SSRs 21 (100%) 18 (95%) 7 (88%) 10 (83%) 56 (93%)
  1. 1Marker are considered scorable when they produced amplicons of less than 500 base pairs
  2. 2 EST-SSRs classified as polymorphic by PolySSR; 3 EST-SSRs classified as monomorphic by PolySSR; along SSRs: at least 10 repeats in repeat motif for dinucleotide SSRs, 6 for tri-SSRs, 5 for tetra-, penta- and hexa-SSRs; bshort SSRs: at most 5 repeats for dinucleotide SSRs, 4 for tri-, tetra-, penta- and hexa-SSRs (see Materials and Methods).