An incremental approach to automated protein localisation

BMC Bioinformatics

Table 2 Numbers of cell masks for the regarded protein locations.

cell compartment	cell masks
cytoplasm including nucleus	144
cytoplasm without nucleus	56
endoplasmic reticulum (ER)	142
lysosomes	222
microtubules	102
mitochondria	268
nucleoli	74
nucleus	150
peroxisomes	71
plasma membrane	97

For each cell compartment a certain number of cell masks was extracted from bright-field images. But in order to realise a protein localisation, an analysis of fluorescence micrographs must be performed. Therefore, each cell mask has been associated with a corresponding region in a fluorescence micrograph that depicts a protein distribution pattern characteristic for this compartment.

ISSN: 1471-2105