Figure 11
Verification of a Corra-identified Ark1 kinase substrate peptide/protein. Following targeted MS/MS identification of the top-ranked Corra-identified discriminatory features (see Figure 10 and Table 2) ion chromatograms were extracted from all LC-MS runs for the peptide RHS*LGLNEAKK (m/z = 444.895 [M+3H]3+), where S* represents phosphoserine. This peptide was derived from the protein YDR293C, and was confirmed as present in all 3 control sample analyses, but absent in all 3 Ark1 knockout analyses, as would be expected. For all six plots, a relative abundance of 100% was manually set to 107 ion counts so that all were on the same scale.