Figure 7
Corra-generated hierarchical clustering of human type 2 diabetes plasma analyses. N-glycosite peptides were isolated from human plasma samples and analyzed via LC-MS, as described under Methods. Randomized, triplicate analyses were performed for each of 22 human plasma samples, 13 controls (NGT: normal glucose tolerance) and 9 from newly diagnosed cases of type 2 diabetes (DB). The hierarchical cluster shown is for the 558 multi-charged features that aligned across all 66 LC-MS runs. Randomly assigned patient numbers are included to show how the replicate MS analyses of the same samples clustered together as the most similar, as expected. One of the misclassified DB patient samples was annotated as from a 'likely not fasted' subject, as required by the OGTT assay used to diagnose diabetes, according to documentation provided with the samples.