Figure 4

An example of the impact of tiny structural variations in the GroES 'mobile loop' that lead to quite dramatic variations in the alignment. A) The 'master-slave' alignments with 1p3h-N as master, variable regions marked at the top, and secondary structure with the mobile loop shown in red at the bottom. B) Alignment matrix with consistency plots along both axes give an overview of variability in each of the alignments from A). C) The different GroEs structures with 1p3h-N in red and 1aon-O and alternatives in blue. Alignment image created using JalView [33] with 'Zappo' colouring; secondary structure assignment according to Xu, et al. [24]. Protein structure rendered using SwissPDBViewer [34] and PovRay [35].