Table 5 Comparison of individual EAs: DART-PCR vs. present paper approach. Individual EAs were obtained during data analyses as reported in Tables 3 and 4.

Target

Dilution

EA individual

Arithmetic mean

SEM

Precision relative error (%)

EA₀ individual

Arithmetic mean

SEM

Precision relative error (%)

Error ratio

SLC6A14 rat

original

1.88, 1.91, 1.80

1.92

0.02

1.1

1.83, 1.86, 1.86

1.91

0.01

0.7

1.59

1 : 4

1.96, 2.00, 1.82

1.91, 1.91, 1.84

1 : 16

1.96, 2.02, 1.90

1.95, 1.92, 1.91

1 : 64

2.03, 1.89, 1.81

1.95, 1.93, 1.96

1 : 256

2.03, 1.87, 1.96

1.97, 2.00, 1.90

SLC22A13 human

original

1.79, 1.85, 1.86

1.70

0.03

1.6

1.94, 1.92, 1.86

1.85

0.02

0.9

1.76

1 : 4

1.78, 1.83, 1.86

1.82, 1.83, 1.89

1 : 16

1.66, 1.61, 1.62

1.80, 1.73, 1.88

1 : 64

1.63, 1.66, 1.62

1.97, 1.87, 1.81

1 : 256

1.65, 1.56, 1.60

1.75, 1.90, 1.82

EMT pig

original

1.83, 1.83, 1.79

1.78

0.02

1.4

1.84, 1.83, 1.82

1.79

0.01

0.4

3.14

1 : 4

1.85, 1.92, 1.64

1.81, 1.79, 1.76

1 : 16

1.68, 1.82, 1.65

1.79, 1.80, 1.78

1 : 64

1.75, 1.94, 1.69

1.82, 1.75, 1.80

1 : 256

1.77, 1.83, 1.72

1.81, 1.75, 1.75

ETT chicken

original

1.95, 1.93, 2.06

1.89

0.02

0.9

1.87, 1.89, 1.87

1.88

0.01

0.4

2.63

1 : 4

1.86, 1.81, 1.97

1.89, 1.88, 1.83

1 : 16

1.87, 1.81, 1.85

1.87, 1.88, 1.82

1 : 64

1.87, 1.92, 1.86

1.92, 1.89, 1.90

1 : 256

1.80, 1.84, 1.86

1.88, 1.91, 1.87

GAPDH human

original

1.86, 1.78, 1.77

1.84

0.01

0.6

2.06, 1.94, 2.02

1.99

0.01

0.5

1.24

1 : 4

1.91, 1.84, 1.82

1.97, 1.99, 2.00

1 : 16

1.80, 1.83, 1.79

1.99, 1.98, 1.97

1 : 64

1.86, 1.86, 1.85

1.96, 1.94, 2.01

1 : 256

1.86, 1.88, 1.83

2.05, 1.97, 1.97