Table 1 Predicted peroxisome-targeted candidates and their experimentally determined localization. (A) PTS2-targeted candidates in mouse. (B) Mouse and human PTS1-targeted candidates taken from a previous publication [15]. RE: regular expression search with perfect match to motif; HMM: Hidden Markov Model profile search; ND: not determined. The PTS2 candidates (Galk2, Qpctl, Sytl3, Fut8, and Ppp3ca) were expressed as fusion proteins with GFP at the C-terminus. The PTS1 candidates KBTBD10 and Zadh2 were expressed as N-terminal GFP fusion proteins.

(A)

PTS2 signal

Galk2

galactokinase 2

RVNIIGEHI

HMM

cytoplasm

Qpctl

glutaminyl-peptide cyclotransferase-like

KLRLVVGQL

HMM

cytoplasm

Sytl3

synaptotagmin-like protein 3-a, isoform a

KLKSHLQHL

HMM+RE

cytoplasm

Fut8

alpha-1,6-fucosyltransferase

RVRVLEEQL

HMM

intracell. organelle (Golgi)

Ppp3ca

protein phosphatase 3, catalytic subunit, alpha isoform

RVDILKAHL

HMM+RE

intracell. organelle/cytoplasm

Ppp3cb

protein phosphatase 3, catalytic subunit, beta isoform

RVDVLKNHL

HMM+RE

ND; low priority clones

Zmiz1

Zinc finger, MIZ-type containing 1

RLQCIKQHL

HMM+RE

Armc6

armadillo repeat containing 6

RLQEVSAHL

HMM+RE

Wdr6

WD repeat domain 6

RVQNLLGHF

HM+RE

ND; low priority clones; PTS2 absent in human orthologs

Adhfe1

Fe-containing alcohol dehydrogenase 1

RVTHLLRHL

RE

Pgm2

phosphoglucomutase 2

KIVTVKTQA

RE

E330021D16Rik

RIKEN cDNA E330021D16 gene

RLRIVSWHL

HMM+RE

ND; protein not expressed

6030452D12Rik

RIKEN cDNA 6030452D12 gene

RLRVIREQL

HMM

ND; no support for protein

2410005O16Rik

RIKEN cDNA 2410005O16 gene

KVEEILAQA

RE

ND; sequence conflict

(B)

PTS1 signal[15]

Zadh2

Zinc binding alcohol dehydrogenase, domain containing 2

SKL

RE

peroxisomes

KBTBD10

kelch repeat and BTB (POZ) domain containing 10

SKL

RE

cytoplasm