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Table 1 Predicted peroxisome-targeted candidates and their experimentally determined localization. (A) PTS2-targeted candidates in mouse. (B) Mouse and human PTS1-targeted candidates taken from a previous publication [15]. RE: regular expression search with perfect match to motif; HMM: Hidden Markov Model profile search; ND: not determined. The PTS2 candidates (Galk2, Qpctl, Sytl3, Fut8, and Ppp3ca) were expressed as fusion proteins with GFP at the C-terminus. The PTS1 candidates KBTBD10 and Zadh2 were expressed as N-terminal GFP fusion proteins.

From: Predicted mouse peroxisome-targeted proteins and their actual subcellular locations

Candidate Description PTS signal Prediction Localization by microscopy
(A) PTS2 signal    
Galk2 galactokinase 2 RVNIIGEHI HMM cytoplasm
Qpctl glutaminyl-peptide cyclotransferase-like KLRLVVGQL HMM cytoplasm
Sytl3 synaptotagmin-like protein 3-a, isoform a KLKSHLQHL HMM+RE cytoplasm
Fut8 alpha-1,6-fucosyltransferase RVRVLEEQL HMM intracell. organelle (Golgi)
Ppp3ca protein phosphatase 3, catalytic subunit, alpha isoform RVDILKAHL HMM+RE intracell. organelle/cytoplasm
Ppp3cb protein phosphatase 3, catalytic subunit, beta isoform RVDVLKNHL HMM+RE ND; low priority clones
Zmiz1 Zinc finger, MIZ-type containing 1 RLQCIKQHL HMM+RE  
Armc6 armadillo repeat containing 6 RLQEVSAHL HMM+RE  
Wdr6 WD repeat domain 6 RVQNLLGHF HM+RE ND; low priority clones; PTS2 absent in human orthologs
Adhfe1 Fe-containing alcohol dehydrogenase 1 RVTHLLRHL RE  
Pgm2 phosphoglucomutase 2 KIVTVKTQA RE  
E330021D16Rik RIKEN cDNA E330021D16 gene RLRIVSWHL HMM+RE ND; protein not expressed
6030452D12Rik RIKEN cDNA 6030452D12 gene RLRVIREQL HMM ND; no support for protein
2410005O16Rik RIKEN cDNA 2410005O16 gene KVEEILAQA RE ND; sequence conflict
(B) PTS1 signal[15]    
Zadh2 Zinc binding alcohol dehydrogenase, domain containing 2 SKL RE peroxisomes
KBTBD10 kelch repeat and BTB (POZ) domain containing 10 SKL RE cytoplasm