Figure 8
From: Impact of T-RFLP data analysis choices on assessments of microbial community structure and dynamics
Similarity versus difference in total fluorescence. Panel A: Analysis of the dilution series dataset. White columns - sample concentration. Gray Columns - average total fluorescence (sum of peak heights) of loading duplicate profiles before treatment as percentage of the average total fluorescence of the undiluted sample loading duplicates. Circles - Jaccard similarity with dilution 1:1. Squares – Bray-Curtis similarity with dilution 1:1. Panel B: Analysis of the DNA-extraction and PCR replicates dataset. Columns – total fluorescence (sum of peak heights) of consensus profiles before normalization as percentage of the highest total fluorescence in the dataset. Circles – Jaccard similarity with sample “Extr. 1 PCR 1&2”. Squares - Bray-Curtis similarity with sample “Extr. 1 PCR 1&2”.