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Table 6 Overview of the generation of the analyzed datasets

From: Impact of T-RFLP data analysis choices on assessments of microbial community structure and dynamics

  Loading duplicates Dilution series DNA-extr. and PCR replicates Time series
Sludge samples     38 samples
DNA-extraction    DNA was extracted 2 times from 1 sludge sample 1 DNA-extraction for each sludge sample
PCR    4 and 2 PCR reactions from the 2 DNA-extraction replicates 2 PCR reactions for each DNA-extraction
Restriction digests   The restriction digest from 1 PCR reaction was diluted to 6 different concentrations. 1 restriction digest per PCR reaction 1 restriction digest per PCR reaction
Gel loading 51 restriction digests were loaded 2 times each 6 restriction digests were loaded 2 times each 1 loading per restriction digest 1 loading per restriction digest
T-RF profiles 102 profiles 12 profiles 2 + 4 profiles 76 profiles
Analyzed pairs 51 pairs 6 pairs 1 + 6 pairs (all possible recombinations of the 4 replicates from the same DNA extraction) 38 pairs