Investigating functional dynamics in heterotrimeric G-proteins. (A) Prediction of large-scale opening motions. (B) Prediction of dynamically coupled sub-domains (colored regions) from correlation network analysis of NMA results. Inter-subdomain couplings are highlighted with thick black lines. (C) Characterization of distinct GTP-active and GDP-inactive states from a clustering of NMA RMSIP results. (D) Fluctuation analysis reveals structural regions with significantly distinct flexibilities (highlighted with a blue shaded background are sites with a p-value < 0.005) between the active (red) and inactive (green) states. Full details for the reproduction of this analysis along with PCA that distinguishes GDP and GTP states can be found in the Additional file 1.