Replicates | Setting 1 | Setting 2 | Setting 3 | Setting 4 | Setting 5 |
---|
2× 2 | 0.382 | 0.387 | 0.388 | 0.413 | 0.917 |
2× 4 | 0.232 | 0.242 | 0.234 | 0.192 | 0.388 |
2× 6 | 0.087 | 0.082 | 0.094 | 0.057 | 0.167 |
2× 8 | 0.006 | −0.009 | 0.007 | 0.015 | 0.038 |
- The table shows the empirical skewness (for calculation see section ‘Methods: Statistics’) yielded for each of the five settings (Table 1) and taking two DNA isolations (‘2×’) with four different exemplary numbers of PCR replicates into account (‘2’, ‘4’, ‘6’, or ‘8’).