Replicates
|
Setting 1
|
Setting 2
|
Setting 3
|
Setting 4
|
Setting 5
|
---|
2× 2
|
0.382
|
0.387
|
0.388
|
0.413
|
0.917
|
2× 4
|
0.232
|
0.242
|
0.234
|
0.192
|
0.388
|
2× 6
|
0.087
|
0.082
|
0.094
|
0.057
|
0.167
|
2× 8
|
0.006
|
−0.009
|
0.007
|
0.015
|
0.038
|
- The table shows the empirical skewness (for calculation see section ‘Methods: Statistics’) yielded for each of the five settings (Table 1) and taking two DNA isolations (‘2×’) with four different exemplary numbers of PCR replicates into account (‘2’, ‘4’, ‘6’, or ‘8’).