Replicates | Setting 1 | Setting 2 | Setting 3 | Setting 4 | Setting 5 |
---|
2× 2 | 0.0% | 0.0% | 0.0% | 0.0% | 0.0% |
2× 4 | 0.0% | 0.0% | 0.0% | 0.0% | 0.0% |
2× 6 | 5.8% | 7.6% | 8.2% | 12.4% | 0.0% |
2× 8 | 84.5% | 8.1% | 73.6% | 4.2% | 2.3% |
- The table shows the results for the simplified χ2 test (for calculation see ‘Methods: Statistics’) yielded for each of the five settings (Table 1) and taking two DNA isolations (‘2×’) with different exemplary numbers of PCR replicates into account (‘2’, ‘4’, ‘6’, or ‘8’).