Figure 1

Main flowchart in BACA. Input: eight gene lists corresponding to four experimental conditions. Up- and down-regulated genes must be included in two separate lists. Step 1: the DAVIDsearch function loads the eight gene lists, queries the DAVID knowledgebase and generates eight different DAVID annotation charts. The red and green boxes evidence the DAVID charts found with up- and down-regulated genes, respectively. Step 2: the BBplot uses the eight DAVID annotation charts to build a plot comparing DAVID annotations across multiple enrichment results. The chart shows a grid where each row represents an enriched annotation found by DAVID and each column the experimental condition where that annotation was highlighted. While, each cell reports a bubble indicating the number of genes enriching the corresponding annotation and the state of these genes in terms of down- and up-regulation.