Distribution of coverage at the individual carrying the singleton alternative allele. We compare the distribution of coverage at called singleton variants between individual-based caller (black) and population-based caller (light gray). The overlap of the two distributions is in dark gray. Here we show all singleton variants after SNP filtering and genotype filtering on quality < 20. We keep individual-based single marker calls at low genotype coverage for this comparison, with the vertical dash line indicating genotype coverage filter at 7x.