Comparison of the sensitivities of RIEMS, Clinical PathoScope, Kraken, and Megablast against the MetaPhlAn clade specific marker database. The plots show the sensitivities calculated from the read to species assignments using the three simulated sample datasets. (A) Our simulated sample comprising 90,385 reads representing original sequences derived from viral, bacterial, and eukaryotic genome sequences (see Table 1). (B) The same dataset as used in (A) but with 5 deviations per read. (C) The Clinical PathoScope simulated sample dataset.