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Table 4 Comparison of RIEMS and EBI metagenomics results

From: RIEMS: a software pipeline for sensitive and comprehensive taxonomic classification of reads from metagenomics datasets

    Assignments by
  16S rRNA reads comprised RIEMS EBI Metagenomics
Class absolute % Reads detected Deviation (basis point) OTUs detected Deviation (basis point)
absolute % absolute %
Bacilli 387 79.3 386 79.1 0.2 27 33.3 46.0
Clostridia 51 10.5 51 10.5 0.0 8 9.9 0.6
Unknown firmicutes - - - - - 2 2.5 2.5
Alphaproteobacteria - - - - - 3 3.7 3.7
Betaproteobacteria 50 10.3 51 10.5 0.2 8 9.9 0.4
Unknown proteobacteria - - - - - 2 2.5 2.5
Unknown bacteria - - - - - 30 37.0 37.0
Assigned    488 100   80 98.7  
Unassigned -   0 0.0   1 1.3  
Total 488 100.0 488 100   81 100  
  1. For this comparison a data subset of 488 16S rRNA sequences comprised in the simulated sequencing dataset was used. The deviation is presented in basis point between the percentage of the respective assignments and the percentage in the original dataset.