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Figure 1 | BMC Bioinformatics

Figure 1

From: aTRAM - automated target restricted assembly method: a fast method for assembling loci across divergent taxa from next-generation sequencing data

Figure 1

Graphic of the aTRAM method. A) Formation of the aTRAM database; DNA is sequenced into a paired-end short read dataset (SRD). aTRAM splits the SRD into shards, creates a BLAST formatted database of the first pair and indexes the paired-end for the sequences in each shard. B) In iteration 0 a query sequence in either amino acid or DNA format is queried against the aTRAM formatted database using BLAST. The top-hits and their paired-ends are selected and assembled de novo. In the following iterations the contigs from the previous iteration are queried against the same database using BLAST, the top-hits and paired-ends selected and assembled de novo until the full locus is assembled.

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BMC Bioinformatics

ISSN: 1471-2105

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