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Table 3 OTUs produced after treating the data with different noise removal approaches

From: NoDe: a fast error-correction algorithm for pyrosequencing amplicon reads

 

Qualitative

Quantitative

Approaches

Total OTUs

Correclt OTUs

Over-splitted OTUs

Missed OTUs

Noisy

Contaminants

Others

Approaches

Rare-OTUs

Redundant OTUs

NoDe

22

17

4

0

1

0

0

NoDe

4

18

AmpliconNoise

29

16

4

1

2

1

5

AmpliconNoise

11

18

Denoiser

24

16

3

1

1

0

4

Denosier

7

17

Pre-cluster

46

17

24

0

4

0

0

Pre-cluster

22

24

Acacia

46

17

23

0

5

1

0

Acacia

21

25

Non denoised

58

17

29

0

5

1

7

Non Denoised

35

17

  1. The left side of the table displays the qualitative OTU assessment and the right side displays the quantitative analysis. For the qualitative analysis, we counted the number of “correct OTUs” (classified as one of the mock species), “noisy OTUs” (classified as one of mock species but only classified until Class, Order or Family level), “missed OTUs” (number of undetected mock species), “over-splitted OTUs” (correct yet redundant classification), “contaminant OTUs” (classified as species no belonging to mock) and “other OTUs” (OTUs unclassified at the Class level or higher). In the quantitative analysis, the number of OTUs with a redundancy below 0.1% (rare OTUs) and the ones with a redundancy above 0.1% (Redundant OTUs) were counted.