Classifier performance on the eleven riboswitches in B. subtilis. Constant length of 157 nt used. Sequence segments chosen according to the scanning procedure. Intergenic regions were divided into non-overlapping 157 nt segments with most downstream segment ending at its corresponding downstream opron. Segments with maximum overlap with riboswitches were chosen as true positives. TP% denotes percentages of correctly classified riboswitches. FP1% denotes percentage of misclassified antisense. FP3% denotes percentage of misclassified negative control segments. Average and standard deviation of the MFE values for the negative control segments are −30.7 and 8.2, respectively.