Figure 4
From: Pydna: a simulation and documentation tool for DNA assembly strategies using python
Outline of the strategy to create a lactose metabolic pathway. Six cloning vectors were constructed from five PCR products and the pYPKa vector linearized using blunt restriction enzymes ZraI, AjiI or EcoRV (A). Two S. cerevisiae expression vectors were formed by homologous recombination between three PCR products and a linearized pYPKpw vector (B and C). The thick blue line represents the linear pYPKpw vector. The two expression cassettes were fused by homologous recombination into a two gene expression vector (D).