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Table 1 The summary of the LC-MS/MS results

From: Comparative proteomics: assessment of biological variability and dataset comparability

Samples Growth phase SpC total a R TS b Number of identified proteins c Total d
   A B C A/B A/C B/C A∩B∩C A∩B A∩C B∩C A B C  
L. lactis JHK24
High-1 expe 2406 2878 4150 1.20 1.72 1.44 221 7 11 20 4 4 17 284
High-2 stat f 4492 4362 4347 1.03 1.03 1.00 262 18 9 6 2 4 2 303
L. lactis HR279
Low-1 exp 3226 2522 4514 1.28 1.40 1.79 233 7 26 21 1 3 17 308
Low-2 stat 3810 4339 4259 1.14 1.12 1.02 242 6 6 43 4 9 5 315
  1. a SpC total is a total number of MS/MS spectra used to identify proteins in sample. False discovery rates (FDR) of peptides were calculated by searching the MS/MS spectra against the forward and the reversed entry database independently. The searching was performed with the 1% of FDR level.
  2. b R TS is the relative number of total spectra.
  3. cThe A, B and C represent the replicates of each sample., A∩B∩C and A∩B represent the number of proteins appeared in all triplicates and two (A and B) out of three replicates, respectively.
  4. dTotal indicated the number of proteins identified from biological replicates. The guideline of protein identification was described in Result section. The number of unique peptide, X! Tandem value and the number presence among replicates were used as a parameter to decide the presence of proteins.
  5. e,fBacterial cell was taken at the early exponential and early stationary phase of cell growth stage, respectively.