Samples
|
Growth phase
|
SpC
total
a
|
R
TS
b
|
Number of identified proteins
c
|
Total
d
|
|---|
| | |
A
|
B
|
C
|
A/B
|
A/C
|
B/C
|
A∩B∩C
|
A∩B
|
A∩C
|
B∩C
|
A
|
B
|
C
| |
|---|
L. lactis
JHK24
|
High-1
| expe
| 2406 | 2878 | 4150 | 1.20 | 1.72 | 1.44 | 221 | 7 | 11 | 20 | 4 | 4 | 17 | 284 |
High-2
| stat f
| 4492 | 4362 | 4347 | 1.03 | 1.03 | 1.00 | 262 | 18 | 9 | 6 | 2 | 4 | 2 | 303 |
L. lactis
HR279
|
Low-1
| exp | 3226 | 2522 | 4514 | 1.28 | 1.40 | 1.79 | 233 | 7 | 26 | 21 | 1 | 3 | 17 | 308 |
Low-2
| stat | 3810 | 4339 | 4259 | 1.14 | 1.12 | 1.02 | 242 | 6 | 6 | 43 | 4 | 9 | 5 | 315 |
-
a
SpC
total
is a total number of MS/MS spectra used to identify proteins in sample. False discovery rates (FDR) of peptides were calculated by searching the MS/MS spectra against the forward and the reversed entry database independently. The searching was performed with the 1% of FDR level.
-
b
R
TS
is the relative number of total spectra.
-
cThe A, B and C represent the replicates of each sample., A∩B∩C and A∩B represent the number of proteins appeared in all triplicates and two (A and B) out of three replicates, respectively.
-
dTotal indicated the number of proteins identified from biological replicates. The guideline of protein identification was described in Result section. The number of unique peptide, X! Tandem value and the number presence among replicates were used as a parameter to decide the presence of proteins.
-
e,fBacterial cell was taken at the early exponential and early stationary phase of cell growth stage, respectively.
