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Fig. 6 | BMC Bioinformatics

Fig. 6

From: Distribution Analyzer, a methodology for identifying and clustering outlier conditions from single-cell distributions, and its application to a Nanog reporter RNAi screen

Fig. 6

Effects of Chek1 and Mediator mRNA depletion on pluripotency. a mRNA changes following shRNA depletion of Chek1 relative to shLuciferase levels. Error bars are mean ± s.d.. b Alkaline phosphatase staining of a Chek1 rescue line in which exogenous doxycycline-inducible expression of Chek1 rescues endogenous depletion. Cells are stained for AP activity following 5 days with (+) and without (−) doxycycline (Dox). Scale bars (in black) represent 200 μm. c Flow cytometry analysis framework, as applied to shLuciferase-transduced NG4 cells. GFP expression of puromycin-resistant mCherry + cells is classified into GFP-, GFP+ and GFP++ categories, representing GFP-negative, GFP-low and GFP-high cell populations, respectively. d Effects of Mediator gene shRNA depletion on NG4 reporter fluorescence distribution. e mRNA changes following shRNA depletion of Med14 and Med27 relative to shLuciferase levels. Error bars are mean ± s.d. f Alkaline phosphatase staining following shRNA depletion of Mediator genes. Scale bars (in black) represent 200 μm

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BMC Bioinformatics

ISSN: 1471-2105

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