Table 4 List of constants used in our reproduced model (compare Table 1)
Lactose operon model parameter list | ||
|---|---|---|
Parameter | Value | Description |
μ | 0.02 m i n −1 | Bacterial growth rate. |
D | 2 m b p | Lac promoter concentration. |
k M | 180 m i n −1 | Maximal transcription initiation rate of the lac promoter. |
k E | 18.8 m i n −1 | Translation initiation rate of lacZ transcript. |
k L | 6.0×104 m i n −1 | Maximal lactose uptake rate per permease. |
γ M | 0.48 m i n −1 | lacZ mRNA dilution/degradation rate. |
γ E | 0.03 m i n −1 | Lac permease degradation/dilution rate. |
γ L | 0.02 m i n −1 | Lactose degradation/dilution rate. |
k pc | 30 | Cooperative promoter-CAP binding site interaction |
p p | 0.127 | Polymerase binding probability to the lac promoter. |
ϕ M | 3.6 ×104 m i n −1 | Max. rate of lac-to-allolac and lac-to-gal metabolism |
K G | 2.6 μ M | CAP complex binding affinity to DNA based on external glucose concentration. |
n h | 1.3 | CAP complex binding affinity to external glucose. |
ξ 2 | 0.05 | Affinity of active repressor for Operator O 2. |
ξ 3 | 0.01 | Affinity of active repressor for Operator O 3. |
ξ 123 | 163 | Stability of the O 1- O 2- O 3-repressor complex. |
ρ max | 1.3 | Repression affinity of lac operon promoter |
K A | 2.92 ×106 m p b | Allolactose-repressor subunit dissociation rate. |
k L | 680 μ M | Half-saturation constant for lactose uptake rate. |
ϕ G | 0.35 | Permease activity as a function of inside glucose concentration |
k G | 1.0 μ M | Permease activity as a function of outside glucose concentration. |
k M | 7.0 ×105 m p b | Max. transcription initiation rate of lac promoter |