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Table 5 Performance on HCV plasmid mixture datasets

From: QQ-SNV: single nucleotide variant detection at low frequency by comparing the quality quantiles

  

QQ-SNVD

QQ-SNVHS-P80 a

LoFreq

ShoRAH

V-Phaser 2

Variant %

Pair

sens.b

FPc

sens.b

FPc

sens.b

FPc

sens.b

FPc

sens.b

FPc

 0.50 %

1d

5/5

10

5/5

38

5/5

36

5/5

2

5/5

11

 0.50 %

2e

0/5

0

0/5

0

0/5

0

0/5

0

3/5

15

 0.50 %

1 + 2f

1/5

0

5/5

0

2/5

5

2/5

7

3/5

32

 1 %

1d

5/5

11

5/5

53

5/5

62

5/5

2

5/5

13

 1 %

2e

1/5

0

5/5

0

5/5

1

2/5

1

2/5

28

 1 %

1 + 2f

3/5

1

5/5

0

5/5

7

4/5

1

3/5

45

 2 %

1d

5/5

13

5/5

64

5/5

72

5/5

4

2/5

20

 2 %

2e

3/5

0

5/5

0

5/5

1

3/5

0

3/5

14

 2 %

1 + 2f

5/5

0

5/5

0

5/5

7

5/5

2

3/5

28

 10 %

1d

5/5

6

5/5

42

5/5

75

5/5

0

3/5

10

 10 %

2e

5/5

0

5/5

0

5/5

0

5/5

0

2/5

28

 10 %

1 + 2f

5/5

0

5/5

0

5/5

8

5/5

1

2/5

56

  

timegh

 

timegh

 

timegi

 

timegj

 

timegj

 

 0.50 %

1d

2m27s

 

2m27s

 

1m30s

 

1h45m

 

8m7s

 

 0.50 %

2e

2m18s

 

2m18s

 

1m33s

 

9h29m

 

22m3s

 

 0.50 %

1 + 2f

4m42s

 

4m42s

 

3m36s

 

15h4m

 

2h31m

 

 1 %

1d

2m56s

 

2m56s

 

2m27s

 

1h38m

 

10m51s

 

 1 %

2e

3m1s

 

3m1s

 

2m6s

 

8h46m

 

46m44s

 

 1 %

1 + 2f

5m48s

 

5m48s

 

5m56s

 

13h13m

 

3h46m

 

 2 %

1d

2m35s

 

2m35s

 

2m6s

 

1h34m

 

10m25s

 

 2 %

2e

2m32s

 

2m32s

 

1m53s

 

8h50m

 

41m1s

 

 2 %

1 + 2f

4m59s

 

4m59s

 

4m34s

 

14h56m

 

1h47m

 

 10 %

1d

3m5s

 

3m5s

 

4m26s

 

1h46m

 

13m45s

 

 10 %

2e

2m30s

 

2m30s

 

2m35s

 

14h13m

 

33m24s

 

 10 %

1 + 2f

5m29s

 

5m29s

 

9m53s

 

7h57m

 

1h47m

 
  1. a80th percentile of the QQ-SNVHS distribution of “error” frequencies was used as frequency cutoff to decrease the number of false positives obtained with QQ-SNVHS
  2. bsens. is the percentage of true variants that were correctly called as SNV
  3. cFP is the number of variants incorrectly called as SNV
  4. dReads 1 of paired-end reads
  5. eReads 2 of paired-end reads (sequenced after paired-end turn)
  6. fAll paired-end reads
  7. gcomputation time in hours (h), minutes (m) and seconds (s)
  8. hWindows 7 64 bit, 8GB RAM, 3.2GHz
  9. iLinux Ubuntu 12.04.4, 57.6 GB RAM, one core (2.3GHz) used
  10. jLinux Ubuntu 12.04.4, 57.6 GB RAM, 8 cores (2.3GHz) used in parallel