Fig. 4
From: FoCo: a simple and robust quantification algorithm of nuclear foci
Simulation of artificial foci images with pre-defined number of foci. a Foci image of a cell 1 h after 10 Gy irradiation. b Foci image of a non-irradiated cell. c Representative simulated foci image with 62 foci (Irradiated in panel (e)). d Representative simulated foci image with 6 foci (Control in panel (e)). e Comparison between pre-defined foci numbers and automatic quantification results. The closer RelDiff 1 to 0, the more precise are the quantification result obtained by the automatic method. f–h Demonstration of quantification results in FoCo, CellProfiler and ImageJ, respectively, applied to the representative image in panel C. i–k Demonstration of quantification results in FoCo, CellProfiler and ImageJ applied to the representative image in panel (d), respectively. Red frames, boundaries and circles designate detected foci in FoCo, CellProfiler and ImageJ, respectively. White arrows designate representative foci, which were either not split by the software or wrongly detected