Fig. 5
From: FoCo: a simple and robust quantification algorithm of nuclear foci
Analysis of images with artificial out-of-focus background signal. a Representative nuclei from base und neighbour images of cells 1 and 6 h after 10 Gy IR and corresponding base images with additional artificial out-of-focus background signal for two different strengths of blur-effect (f = 1, 6). b Relative differences RelDiff 2 for images of cells 1 and 6 h after 10 Gy IR. Quantifications were performed in ImageJ, CellProfiler and FoCo. The lower RelDiff 2 , the more robust is the method in sense of suppressing out-of-focus background signal