Fig. 1
From: misFinder: identify mis-assemblies in an unbiased manner using reference and paired-end reads
Workflow of misFinder. MisFinder consists of three major steps. (1) Identify putative mis-assembles. Scaffolds (contigs) and genome reference are first used to generate the BLASTN alignments followed by the alignment processing that the redundant alignments will be removed, and then the putative mis-assembles are identified according to their non-redundant alignments. (2) Breakpoint computation. Paired-end reads are aligned to the scaffolds to make the breakpoints more accurate. (3) Mis-assembly validation. Putative mis-assemblies are validated according to the alignment information of paired-end reads on scaffolds