Fig. 3

Isotype distribution related to flow cytometric index data. Upper panel: Distribution of IgG isotypes for all cells for which paired Ig heavy and light chain sequences could be determined. The data of each donor is split into two categories according to whether sciReptor identified an associated kappa or lambda chain. Lower panels: Indexed flow cytometry data of the sorted cells that were subjected to the sequencing process (gray dots). During the single-cell isolation, the cells were labeled with anti-Ig κ and anti-Ig λ antibodies (conjugated to PE-Cy7 and PE, respectively), whose respective fluorescence intensity is plotted. The cells for which Ig heavy and light chain sequences could be obtained are additionally color-coded according to the identified IgG isotype