Fig. 7

Titration Response. To examine the titration response, we plot the proportion of features that show monotone increasing expression as the amount of input RNA increases stratified by the difference in expression between the sample being titrated and the sample being held constant. Here we use samples 2–4 and 6–8 as two separate titration series. To assess the difference in expression between mixture components A and B, we use the expression estimates in the pure sample types: sample type 1 (pure A) and sample type 5 (pure B). Each point represents an average over the miRNAs falling within a given bin, where bins are defined based on the observed difference in pure sample expression (x-axis). The The Life Technologies (LifeTech) values are plotted with solid circles; the qpcR values with open squares