Fig. 1

Flowchart illustrating the workflow of LimiTT. The input (grey) is composed of an optional list of miRNAs and an optional annotation file with a transcriptome/proteome. If an annotation file was submitted, the black path represents the processing steps of LimiTT, otherwise, the process is described by the red path. a The workflow starts with the selection of miRNA target interactions (MTIs) from the four MTI databases (DBs) in consideration of the miRNAs supplied by the user. Additionally the MTI stringency can be filtered by adjusting several parameters. b All target gene symbols of the selected MTIs are mapped to UniProt Accessions (UniProtAccs), while c all UniProtAccs are filtered from the annotation file simultaneously. d Subsequently, both lists are overlapped, resulting in those MTIs which can be linked to the submitted data. If no annotation file is provided, steps (c) and (d) are ignored, and the resulting MTIs rely on the miRNA list or just on the adjustable properties. e Optionally, an enrichment analysis of the identified MTI sets can be performed based on a ranked list with UniProtAccs supplied by the user