Fig. 1
From: flowVS: channel-specific variance stabilization in flow cytometry
Mean fluorescence intensities (MFIs) of one-dimensional cell populations (also called density peaks) are plotted against the variances of the populations. Blood samples were collected from five healthy individuals on different days and stained with labeled antibodies against five biomarkers (see Section 3). Samples are compensated and gated for the lymphocytes, but no transformation is used. Populations identified in each fluorescence channel are shown with the same symbol and color. We observe that without proper transformation, variance increases monotonically with MFI