Fig. 3

Ortholog, paralog and population genomic data are highly complementary. a Venn diagram for three different data sets using a definition of ω < 0.6. As most genes with evidence of negative selection are unique to one specific data set, this indicates that all three data sets are highly complementary. b Venn diagram for three different data sets using a definition of ω < 1 and P < 0.05. Again, most genes with evidence of negative selection are unique to one specific data set. c Overlap between different thresholds to define negative selection and expressed genes. d Identification of a candidate gene set of orphan genes that lack any expression data, are under significant negative selection and also have ω value less than 0.6 in at least two of the three datasets