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Table 3 Summary of results from the three analyses of structure-consistent protein alignments, using PsychoProt with standard parameters and both descriptor sets 1 (physicochemical properties) and 2 (metabolic descriptors)

From: Detection and sequence/structure mapping of biophysical constraints to protein variation in saturated mutational libraries and protein sequence alignments with a dedicated server

Protein TEM-1β-lactamase BcII metallo-β-lactamase Aquaporin-0
Soluble/Membrane and oligomerization state Soluble monomer Soluble monomer Transmembrane tetramer
PDB ID 1XPB 1BC2 1SOR
Source of alignment From EVFold server From EVFold server From Ovchinnikov et al. eLife [25]
Sites explained by some descriptor 61.6 % 69.6 % 61.1 %
Hydrophobicity 47.5 % 48.7 % 52.6 %
 Two metabolic descriptors 39.5 % 27.8 % 17.0 %
Volume 5.6 % 11.4 % 15.6 %
Unexplained of very low tolerance (k* < 4) 2.7 % 4.4 % 10.4 %
Unexplained of very high tolerance (k* > 16) 0 % 0.9 % 0 %
Unexplained of not extreme tolerance to substitutions 35.7 % 25.1 % 28.5 %