Fig. 2

Benchmarking against the immunopathology of the metastatic melanomas: a p value heatmap and boxplots representing pairwise t –tests for all clinical features and their patient groups. The darkest shade of blue illustrates p values < 0.05 (the lighter shade of blue represents p values >0.05 and < 0.1). Most notably, for p values < 0.05, there was a significant difference between those patient groups positive for a pathological CD3+ brisk infiltrate compared to the patients which were absent for CD3+ brisk. A comparison is labeled dark blue if at least one DIST in the patient group comparison was significant. The three boxplots pointing to that comparison illustrates distinct phenotypes of CD8+ T cells [38, 39], which contribute to the T cell pathology observed in the brisk-positive group. b Hierarchical clustering and heatmap (rows represent tumor samples and columns DISTs), across all patients and a comprehensive set of DISTs from several general immune-cell types. The heatmap illustrates clusters of tumor samples that have elevated immune-cell presence in their tumor, and a cluster of patient that are less active for DIST signatures. c Kaplan-Meier curves illustrating the three DIST types that correspond to positive outcome in the log-rank difference between the patient groups which were from the highest quartile (green), and the lowest quartile (black) for the DIST signature score. Patients with the highest quartile signal in their tumors for naïve CD8+ T cells [38], central memory Th1 cells [40], mature inactivated NK cell population of cells [38], and regulatory CD19+ B cells [44] were predictive of positive outcome