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Table 1 Selected functions. The table lists a subset of over 50 methods and functions defined by systemPipeR. Usage instructions are provided in the corresponding help pages and vignettes of the package

From: systemPipeR: NGS workflow and report generation environment

Function name

Description

genWorkenvir

Generates workflow templates provided by systemPipeRdata helper package

systemArgs

Constructs SYSargs workflow control module (S4 object) fromtargets and param files

runCommandline

Executes command-line software on samples and parameters specified in SYSargs

clusterRun

Runs command-line software in parallel mode on a computer cluster

preprocessReads

Filtering and/or trimming of short reads using predefined or custom parameters

seeFASTQ/seeFASTQplot

Generates quality reports for any number of FASTQ files

alignStats

Generates alignment statistics, such as total number of reads and alignment frequency

run_edgeR/run_DESeq2

Runs edgeR or DESeq2 for any number of pairwise sample comparisons

filterDEGs

Filters and plots DEG results based on user-defined parameters

overLapper/vennPlot

Computation of Venn intersects for 2-20 or more samples and 2-5 way Venn diagrams

GOCluster_Report

GO term enrichment analysis for large numbers of gene sets

variantReport

Generates a variant report containing genomic annotations and confidence statistics

predORF

Prediction of short open reading frames in DNA sequences

featuretypeCounts

Computes and plots read distribution for many feature types at once

featureCoverage

Computes and plots read depth coverage from many transcripts