Fig. 4
From: FMAP: Functional Mapping and Analysis Pipeline for metagenomics and metatranscriptomics studies
Performance of FMAP in real datasets. a Boxplots of percent mapping rates of FMAP using DIAMOND using four reference libraries, including the following: COG (2003) used by ShotgunFunctionalizeR, KEGG (v54) used by HUMAnN, KFU used by FMAP, and RefSeq (07/2014) used by MEGAN. b Boxplots of differences in mapping rates of COG, KEGG and RefSeq compared to the KFU. The values are the percent of reads mapped by KFU that are greater than the comparison database shown in A. The points indicate the values for samples from SRP002423 (▲), SRP050543 (▼), and SRP044400 (●). Boxplots drawn to represent the 25th and 75th percentiles (the lower and upper quartiles, respectively) as a box with a band in the box representing 50th percentile (the median). The upper whisker is located at the ‘smaller’ of the maximum x value and 3rd quartile + 1.5 inner quantile range (IQR), whereas the lower whisker is located at the ‘larger’ of the smallest x value and 1st quartile - 1.5 IQR. c Heatmap of differentially abundant genes of SRP002423. The samples were clustered by the genes into the two groups, healthy control (HC) and Crohn’s disease (CD)