Fig. 3

Plot showing conformational differences in Tubb2b ^A248V and Tubb2b ^R380L. Outermost layer indicates reported SNVs (gray, not validated; red, non-synonymous; green, synonymous) from dbSNP. a. Conservation scores represented as a histogram. These values are generated as described in “ MODICT methodology” section and are not related to MODICT score. b and c. Amino acid sequences with residues colored according to their property (positively charged, red triangle; negatively charged, blue triangle, non-polar, gray circle; polar, pink circle; aromatic ring, green hexagon). d. Iterative MODICT scores of individual residue pairs (algorithm, Eq. 1) resulting from comparison with Tubb2b ^WT. Top layer belongs to Tubb2b ^A248V whereas bottom layer belongs to Tubb2b ^R380L. Each blue histogram bin designates the contribution of a residue pair to the overall MODICT score (Higher bars mean more contribution as well as more the adverse effect of that residue pair on structural stability). These histogram bins are generated by iterative MODICT algorithm and are colored according to conservation. e. Important regions, SNVs and Indels are marked with boxes