Convert your favorite protein modeling program into a mutation predictor: “MODICT”

BMC Bioinformatics

Table 1 Mutations in PAH

Mutation	Residual	Score
	activity (%)	(Higher means more deleterious)
Y414C	28	0.112
R241C	25	0.136
A403V	32	0.125
R261Q	30	0.071
E390G	75	0.086
R68S	98	0.157
I65T	29	0.153
V245A	50	0.126
L48S	39	0.247
F39L	96	0.136
D415N	72	0.072
A395P	15	0.139
A104D	26	0.091
R408Q	55	0.063
P211T	72	0.185
V388M	43	0.15
R241H	23	0.131
I306V	39	0.161

Mutations in PAH with their residual enzyme activity and modict scores are listed. The values listed are expressed in percentages of residual enzyme activity with respect to wildtype enzyme activity. modict scores are generated taking into account the catalytic domain (143–410; [54]). As outlined in “Results” section, when more than or equal to 3 mutations with enzymatic activities are present, the first method can be used where the correlation between the textscmodict scores and the enzymatic activities are measured. Since higher modict scores are more deleterious, one would expect a negative correlation between modict scores and enzyme activity. The interpretation of these results are explained in more detail in Fig. 6

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