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Fig. 1 | BMC Bioinformatics

Fig. 1

From: Genome-wide functional analysis using the barcode sequence alignment and statistical analysis (Barcas) tool

Fig. 1

Barcode-seq data. a Barcode DNAs of shRNAs. Sense or anti-sense strands of shRNAs are generally used for barcodes (barcode sequences and shRNA sequences are the same.). However, sometimes, barcodes are separately inserted to identify shRNAs (barcode sequences and shRNA sequences are different.), such as the Cellecta library; b Barcode DNAs of yeast deletion strains. For a gene, there are two barcodes (‘UP’ and ‘DN’) in yeast deletion strains; c Two read formats for single-tag and pair-tag in barcode-seq data. In a barcode sequence region, it also contains a universal primer sequence

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